[1]段海潇,杨俊寒,王琳,等.表达近红外荧光蛋白的结肠癌细胞系的建立[J].湖北工业大学学报,2020,(5):71-74.
　DUAN Haixiao,YANG Junhan,WANG Lin,et al.Construction of Colorectal Cancer Model with the Stable Expression of iRFP720[J].,2020,(5):71-74.
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表达近红外荧光蛋白的结肠癌细胞系的建立()
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《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
期数:
2020年第5期
页码:
71-74
栏目:
湖北工业大学学报
出版日期:
2020-10-28

文章信息/Info

Title:
Construction of Colorectal Cancer Model with the Stable Expression of iRFP720
文章编号:
1003-4684(2020)05-0071-04
作者:
段海潇; 杨俊寒; 王琳; 王润杨;  张紫怡; 邹宗幸;  胡翰;  汪洋;  刘滨磊
湖北工业大学生物工程与食品学院， 湖北 武汉 430068
Author(s):
DUAN Haixiao; YANG Junhan; WANG Lin; WANG Runyang;  ZHANG Ziyi;   ZOU Zongxing;  HU Han; WANG Yang ; LIU Binlei
School of Biological Engin. and Food Science, Hubei Univ., of Tech .Wuhan 430068, China
关键词:
CT26;  iRFP720;  细胞株;  荧光成像
Keywords:
CT26;  iRFP720;  cell lines;  Fluorescence imaging
分类号:
R153
文献标志码:
A
摘要:
通过PiggyBac转座子系统,构建能够稳定表达近红外荧光蛋白iRFP720的CT26鼠结肠癌细胞株，通过嘌呤霉素筛选获得CT26-iRFP720单克隆细胞株。流式细胞仪验证表明所得CT26-iRFP720细胞株纯度为98.1%；Western Blot检测结果表明，iRFP720基因的引入不会改变细胞PD-L1的表达。将构建成功的细胞株植入BALB/c小鼠皮下，成功建立CT26-iRFP720肿瘤模型，且利用动物活体成像可以观察到iRFP720的表达。结果表明稳定，可实时观察的CT26-iRFP720结肠癌BALB/c小鼠模型成功建立。
Abstract:
To construct a CT26 iRFP720 cell line with stable expression of near infrared fluorescent protein iRFP720 and resistance, CT26 iRFP720 monoclonal cell line was obtained by puromycin selection. The purity of CT26 iRFP720 monoclonal cell line was 98.2% by flow cytometry detection. At the same time, western blot showed that the introduction of iRFP 720 gene did not change the expression of PD L1. CT26 iRFP720 tumor model was successfully established by implanting subcutaneously the constructed cell line into the right flank of BALB/c mice, and the expression of iRFP720 could be observed by in vivo Fluorescence imaging. The results showed that a stable and real time observed model of CT26 iRFP720 colon cancer in BALB/c mice was successfully established.

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备注/Memo

备注/Memo:
［收稿日期］ 2020-02-27
［第一作者］ 段海潇(1993-)， 男， 山西晋城人，湖北工业大学硕士研究生，研究方向为生物化工
［通信作者］ 刘滨磊(1962-)， 男， 湖北武汉人，湖北工业大学教授，研究方向为生物制药
更新日期/Last Update: 2020-10-28