[1]李雨莹,罗凯伦,邹宗幸,等.荧光素酶表达的脑胶质瘤小鼠模型的建立[J].湖北工业大学学报,2020,(5):75-77.
　LI Yuying,LUO Kailun,ZOU Zongxing,et al.Construction of a Mouse Model of Glioma Capable of Expressing Luciferase[J].,2020,(5):75-77.
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荧光素酶表达的脑胶质瘤小鼠模型的建立()
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《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
期数:
2020年第5期
页码:
75-77
栏目:
湖北工业大学学报
出版日期:
2020-10-28

文章信息/Info

Title:
Construction of a Mouse Model of Glioma Capable of Expressing Luciferase
文章编号:
1003-4684(2020)05-0075-03
作者:
 李雨莹;  罗凯伦;  邹宗幸;  刘滨磊;  汪  洋
湖北工业大学生物工程与食品学院， 湖北 武汉 430068
Author(s):
LI Yuying;  LUO Kailun;  ZOU Zongxing;  LIU Binlei;  WANG Yang
School of Biological Engin. and Food Science, Hubei Univ., of Tech .Wuhan 430068, China
关键词:
U87MG;  荧光素酶;  生物发光;  细胞株
Keywords:
U87MG;  luciferase;  bioluminescence;  cell line
分类号:
R73
文献标志码:
A
摘要:
运用PiggyBac转座子系统，通过嘌呤霉素筛选构建稳定表达荧光素酶的U87MG细胞株（U87MG-FLUC）。通过流式细胞术检测U87MG-FLUC细胞株纯度达到100%。该细胞系传代后能稳定表达荧光素酶，且生物发光的强度与细胞个数成正比。将该稳定表达荧光素酶的细胞接种于免疫缺陷型小鼠的颅内，肿瘤接种后的7 d，利用活体成像仪对植瘤小鼠进行检测，确定肿瘤的大小以及位置。结果表明：接种了U87MG-FLUC细胞的裸鼠，均长出肿瘤；成功构建了表达荧光素酶的U87MG细胞株，且人脑胶质瘤细胞系的裸鼠肿瘤模型构建成功。
Abstract:
U87-MG cell line (U87MG-FLUC) stably expressing luciferase was constructed by puromycin selection using the PiggyBac transposon system. U87MG-FLUC cell line was 100% pure by flow cytometry; the cell line was able to stably express luciferase after passage, and the intensity of bioluminescence was directly proportional to the number of cells. The cells stably expressing luciferase were inoculated into the skull of immunodeficient mice. Seven days after tumor inoculation, tumor-implanted mice were detected using a live imager to determine the size and location of the tumor. Nude mice inoculated with U87MG-FLUC cells developed tumors. The results showed that the U87MG cell line expressing luciferase was successfully constructed, and a nude mouse tumor model of a human glioma cell line was successfully constructed.

参考文献/References:

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相似文献/References:

[1]王 琳,张紫怡,金 静,等. 稳定表达荧光素酶BGC823细胞系的构建[J].湖北工业大学学报,2021,(4):42.
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备注/Memo

备注/Memo:
［收稿日期］ 2020-03-12
［第一作者］ 李雨莹(1993-)，女，湖北省宜昌市人，湖北工业大学硕士研究生，研究方向生物医药
更新日期/Last Update: 2020-10-28