[1]毛亚浩,丁静怡,余 君,等. 基于OAV和PCA的茄芯烟叶中关键呈香物质[J].湖北工业大学学报,2023,(1):47-52.
 MAO Yahao,DING Jingyi,YU Jun,et al. Analysis of Key Aroma Compounds in Filler Leaves based on OAV and PCA[J].,2023,(1):47-52.
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 基于OAV和PCA的茄芯烟叶中关键呈香物质()
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《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
期数:
2023年第1期
页码:
47-52
栏目:
湖北工业大学学报
出版日期:
2023-03-13

文章信息/Info

Title:
 Analysis of Key Aroma Compounds in Filler Leaves based on OAV and PCA
文章编号:
1003-4684(2023)01-0047-06
作者:
 毛亚浩1 丁静怡1 余 君2 王 志1 陈 雄1 杨春雷2 姚 兰1
 1 湖北工业大学 生物工程与食品学院, 发酵工程教育部重点实验室, 湖北 武汉 430068;
 2 湖北省烟草科学研究院, 湖北 武汉 430030
Author(s):
 MAO Yahao1 DING Jingyi1 YU Jun1 WANG Zhi1 CHEN Xiong2 YANG Chunlei2 YAO Lan1
 1 Key Laboratory of Fermentation Engin.,Ministry of Education,Hubei Univ. of Tech., Wuhan 430068,China;
 2 Hubei Provincial Key Laboratory of Industrial Microbiology,Hubei Univ. of Tech., Wuhan 430068, China;
关键词:
 茄芯烟叶 中性香气 气味活性值 主成分分析
Keywords:
 Cigar filler leaves Neutral aroma OAV PCA
分类号:
TS453
文献标志码:
A
摘要:
 为探究恩施茄芯烟叶中性香气成分,筛选关键呈香物质,以晾制结束后的恩施茄芯烟叶作为研究对象,分别做了两组处理:无菌水发酵处理、Bacillus megaterium m1加培养基混合发酵处理,利用GC-MS鉴定茄芯烟叶中的中性香气成分,通过气味活性值(OAV)与主成分分析(PCA)相结合筛选其关键致香成分。结果表明:从茄芯烟叶共鉴定出30种中性致香成分,包括酮类11种、醇类5种、酯类1种、杂环类2种、烯烃类1种、醛类8种、酚类2种,其中酮类种类较丰富,烯烃类含量较高。挥发性成分中有11种OAV大于1,最终确认苯甲醛、4-乙烯基愈创木酚、苯乙醛、巨豆三烯酮1、巨豆三烯酮2为恩施茄芯烟叶中性香气成分的关键呈香物质。这为解析恩施茄芯烟叶的特征性香味风格与品质定向调控提供了理论基础。
Abstract:
 In order to screen the key aroma substances of cigar filler leaves from Enshi, two groups of treatments on the cigar filler leaves after drying were performed: sterile water, or Bacillus megaterium m1 plus the medium was mixed evenly with cigar filler leaves and then fermented for 18 days. The neutral aroma components in cigar filler leaves were determined by GC-MS, and the key aroma components were screened by the combination of Odor Activity Value (OAV) and Principal Component Analysis (PCA). The result showed that a total of 30 neutral aroma components were detected from cigar filler leaves, including 11 ketones, 5 alcohols, 1 ester, 2 heterocycles, 1 olefin, 8 aldehydes, and 2 phenols Ketones are more abundant than other types of components, and olefins are the highest in content. Among the volatile components, 11 kinds of OAVs are greater than 1. Finally, benzaldehyde, 4-vinylguaiacol, phenylacetaldehyde, megastigmatrienon1 and megastigmatrienon 2 were selected as the key aroma compounds of neutral aroma components in cigar filler leaves from Enshi. The results of this study provide a theoretical basis for analyzing the characteristic aroma style and quality-oriented regulation of Enshi cigar filler leaves.

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备注/Memo

备注/Memo:
[收稿日期] 2022-08-30
[基金项目] 国家自然科学基金(31871789);中国烟草总公司重大专项项目(110202001039(XJ-01));湖北省烟草公司重点科技项目(027Y2019-003)
[第一作者] 毛亚浩(1995-),男,河南平顶山人,湖北工业大学硕士研究生,研究方向为发酵工程
[通信作者] 姚 兰(1982-),女,四川南充人,湖北工业大学副教授,研究方向为功能酵母与酿造微生物
更新日期/Last Update: 2023-03-14