[1]陈安利,陈艳熙,马 卓. 茯苓枸杞饮提取工艺及其对黄嘌呤氧化酶作用[J].湖北工业大学学报,2022,(5):71-75.
　CHEN Anli,CHEN Yanxi,MA Zhuo. Study on Extraction Technology of Poria Cocos and Medlar Drink and Its Effect on Xanthine Oxidase[J].,2022,(5):71-75.
点击复制

 茯苓枸杞饮提取工艺及其对黄嘌呤氧化酶作用()
分享到：

《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
期数:
2022年第5期
页码:
71-75
栏目:
湖北工业大学学报
出版日期:
2022-10-25

文章信息/Info

Title:
 Study on Extraction Technology of Poria Cocos and Medlar Drink and Its Effect on Xanthine Oxidase
文章编号:
1003-4684(2022)05-0071-05
作者:
 陈安利;  陈艳熙;  马 卓
 湖北工业大学生物工程与食品学院， 湖北 武汉 430068
Author(s):
 CHEN Anli;  CHEN Yanxi;  MA Zhuo
 College of Bioengineering and Food, Hubei Univ. of Tech., Wuhan 430068, China
关键词:
 ; 茯苓;  枸杞;  黄嘌呤氧化酶;  抑制作用
Keywords:
 ; poria cocos;  lycium barbarum;  xanthine oxidase;  inhibitory effect
分类号:
TS275.5
文献标志码:
A
摘要:
 为了研发一种对痛风具有预防作用的保健产品，以茯苓、枸杞等药食同源中药材为主要原料，以多糖得率和干膏得率为评价指标，采用正交实验对茯苓枸杞饮的加水倍数、煎煮时间和煎煮次数进行优化。检测茯苓枸杞饮与苯溴马隆对黄嘌呤氧化酶（XOD）活性的体外抑制作用，通过紫外分光光度法确定最佳检测条件。结果表明，最佳水提工艺为加8倍水、浸提3次、每次90 min，重复性实验证明，按此工艺参数，可制备品质稳定的茯苓枸杞饮。且在290 nm处、酶浓度200 μg/mL条件下，茯苓枸杞饮相对于苯溴马隆，对XOD一定的抑制作用，且抑制率为（43.79±0.685）%，IC50为（87.25±0.254）mg/mL。该制备工艺设计合理，操作简单可行，且对XOD有一定的抑制作用，可起到预防痛风的作用，可为生产提供可靠试验依据。
Abstract:
 Objective of this paper is to develop a health care product with preventive effect on gout. By taking Poria cocos, Lycium barbarum and other Chinese medicinal materials with homologous medicine and food as main raw materials, taking polysaccharide yield and dry extract yield as evaluation indexes, orthogonal experiment was used to optimize the water addition multiple, decoction time and decoction times of Poria cocos Lycium barbarum drink. The inhibitory effects of Fuling Lycium barbarum Decoction and benzbromarone on xanthine oxidase (XOD) activity in vitro were detected, and the best detection conditions were determined by ultraviolet spectrophotometry. The results showed that the best water extraction process was to add 8 times of water, extract for 3 times, each time for 90 minutes. The repeatability experiment proved that according to this process parameter, Poria cocos Lycium barbarum drink with stable quality could be prepared. And at 290 nm, under the condition of enzyme concentration of 200 g/mL, Fuling Lycium barbarum drink has a certain inhibitory effect on XOD compared with benzbromarone, and the inhibitory rate is 43.79±0.685%，IC50 is 87.25±0.254mg/mL; Conclusion the preparation process is reasonable in design, simple and feasible in operation, and has a certain inhibitory effect on XOD, which can prevent gout and provide reliable experimental basis for production.

参考文献/References:

[1] BUPPARENOO P, PAKCHOTANON R, NARONGROEKNAWIN P, et al. Effect of curcumin on serum urate in asymptomatic hyperuricemia: a randomized placebo-controlled trial[J]. Journal of Dietary Supplements, 2020(6):1-13.
[2] 李安,肖小年.黄嘌呤氧化酶抑制剂研究进展及发展前景[J].江西食品工业,2012，48 (1):43- 45.
[3] 王琤.高尿酸血症和痛风患者用药有讲究[J].开卷有益[CD*2/3]求医问药,2021(2):16-18.
[4] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社, 2020:240-241.
[5] 田婷,陈华,殷璐,等.茯苓和茯苓皮水和乙醇提取物的利尿作用及其活性成分的分离鉴定[J].中国药理学与毒理学杂志,2014,28(1):57-62.
[6] LEE S R, LEE S, MOON E,et al. Bioactivity-guided isolation of anti-inflammatory triterpenoids from the sclerotia of Poria cocos using LPS-stimulated Raw264.7 cells[J]. Bioorganic Chemistry, 2016, 70(Complete):94-99. 
[7] 梁丹灵. 树舌灵芝和茯苓提取物改善高尿酸血症作用研究[D].广州：广州中医药大学,2019.
[8] 郭花斌,辛宝.药食两用中草药在防治高尿酸血症上的研究进展[J].世界最新医学信息文摘,2018,18(86):50-51,53. 
[9] 张喜康,赵宇慧,刘军,等.枸杞不同生长期多糖的理化特性及结构分析[J].食品科学,2020,41(16):158-164.
[10] WU D T, GUO H,SHANG L, et al. Review of the structural characterization, quality evaluation, and industrial application of Lycium barbarum polysaccharides[J]. Trends in Food Science & Technology，2018，79：171-183. 
[11] 侯学谦,祝婉芳,曲玮,等.枸杞化学成分及药理活性研究进展[J].海峡药学,2016,28(8):1-7.
[12] 刘亚飞,彭新颜,贺红军,等.桑葚浓缩汁对D-半乳糖诱导氧化损伤大鼠脾脏的保护作用[J].食品科学,2018,39(1):192-199.
[13] CHEN C,YOU L J, ABBASI A M, et al. Optimization for ultrasound extraction of polysaccharides from mulberry fruits with antioxidant and hyperglycemic activity in vitro[J]. Carbohydrate Polymers, 2015, 130:122-132. 
[14] 魏晓楠,郝铁成.中药提取新技术研究进展[J].中国野生植物资源,2020,39(9):47-50.
[15] 邓耒娇,闫洁熙,王沛,等.茯苓多糖对高尿酸血症大鼠肾小管转运体rURAT1、rOAT1和rOCT2表达的影响[J].西部中医药,2019,32(6):10-14.
[16] 罗春萍,陆友利,王星星.苯酚[CD*2/3]硫酸法快速测定多糖方法的优化[J].化工管理,2021(3):90-91,94.
[17] 赵婷婷,何承辉,谭梅娥,等.基于多指标权重分析和单因素[CD*2/3]正交实验优选前列爽颗粒醇提工艺[J].化学与生物工程,2020,37(12):45-50.
[18] 方天仪,李曼曼,丁雪东,等.16种中药水提物和醇提物对黄嘌呤氧化酶的抑制作用[J].黑龙江畜牧兽医,2019(7):112-115.

相似文献/References:

[1]熊韧,曹海印,王焱清,等.非牛顿润滑静压轴承的节流器流量方程修正[J].湖北工业大学学报,2019,34(5):6.
　XIONG Ren,CAO Haiyin,WANG Yanqing,et al.Modified restrictor flow equations of hydrostatic bearings ubricated by non-Newtonian fluids[J].,2019,34(5):6.
[2]王照远,曹 民,王 毅,等. 场景与数据双驱动的隧道图像拼接方法[J].湖北工业大学学报,2020,(4):11.
　WANG Zhaoyuan,CAO Min,WANG Yi,et al. Tunnel Image Stitching Method based on Scene and Data[J].,2020,(5):11.
[3]潘 健,梁佳成,陈凤娇,等. 单电流闭环多重PR控制的LCL型逆变器[J].湖北工业大学学报,2020,(4):16.
　PAN Jian,LIANG Jiacheng,CHEN Fengjiao,et al. Design of LCL Grid Connected Inverter based on Single Closed Loop Control and Multiple PR Controllers[J].,2020,(5):16.
[4]王晓光,赵 萌,文益雪,等. 定子闭口槽结构对永磁电机齿槽转矩影响分析[J].湖北工业大学学报,2020,(4):25.
　WANG Xiaoguang,ZHAO Meng,WEN Yixue,et al. Study on Cogging Torque and Vibration Noise of Permanent Magnet Motor with Segmental Stator and Closed-Slot[J].,2020,(5):25.
[5]宇 卫,凃玲英,陈 健. 风电场集中接入对集电线电流保护的影响[J].湖北工业大学学报,2020,(4):29.
　YU Wei,TU Lingying,CHEN Jian. Effect of the Collective Line Current Protection when Wind Farms are Centralized Accessed to the Power System[J].,2020,(5):29.
[6]廖政斌,王泽飞,祝 珊. 二惯量系统谐振在线抑制及相位补偿[J].湖北工业大学学报,2020,(4):34.
　LIAO Zhengbin,WANG Zefei,ZHU Shan. Online Resonance Suppression and Phase Compensation for Double Inertia System[J].,2020,(5):34.
[7]王 欣,游 颖,姜天翔,等. 面向3D打印过程的产品工艺设计和优化[J].湖北工业大学学报,2020,(4):39.
　WANG Xin,YOU Ying,JIANG Tianxiang,et al. Product Process Design and Optimization for 3D Printing Processes[J].,2020,(5):39.
[8]冉晶晶,文 红,罗雅梅,等. 全自动样品前处理平台及其控制系统[J].湖北工业大学学报,2020,(4):43.
　RAN Jingjing,WEN Hong,LUO Yamei,et al. Research on Automatic Sample Preprocessing Platform and its Control System[J].,2020,(5):43.
[9]杨 磊,马志艳,石 敏,等. 基于模糊PID的小型冷库过热度控制方法[J].湖北工业大学学报,2020,(4):43.
　YANG Lei,MA Zhiyan,SHI Min,et al. Research on Superheat Control Method of Small Cold Storage based on Fuzzy PID[J].,2020,(5):43.
[10]黄 晶,周细枝,周业望. 动态注塑成型模具的设计与实验研究[J].湖北工业大学学报,2020,(4):52.
　HUANG Jing,ZHOU Xizhi,ZHOU Yewang. Design and Experimental Study of Dynamic Injection Molding[J].,2020,(5):52.

备注/Memo

备注/Memo:
［收稿日期］ 2021-07-21
［第一作者］ 陈安利（1996-），女，湖北襄阳人，湖北工业大学硕士研究生，研究方向为中药制剂
［通信作者］ 马 卓（1962-），女，湖北武汉人，湖北工业大学教授，研究方向为中药制剂
更新日期/Last Update: 2022-10-26