[1]严子云,陈 雄,李 欣. 响应面法优化巴氏醋杆菌的耐酸醇营养盐培养[J].湖北工业大学学报,2021,(2):39-45.
　YAN Ziyun,CHEN Xiong,LI Xin. Response Surface Methodology to Optimize the Nutrient Medium of Acetobacter Pasteurianus[J].,2021,(2):39-45.
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 响应面法优化巴氏醋杆菌的耐酸醇营养盐培养()
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《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
期数:
2021年第2期
页码:
39-45
栏目:
湖北工业大学学报
出版日期:
2021-04-22

文章信息/Info

Title:
 Response Surface Methodology to Optimize the Nutrient Medium of Acetobacter Pasteurianus
文章编号:
1003-4684(2021)02-0039-07
作者:
 严子云;  陈 雄;  李 欣
 发酵工程教育部重点实验室，湖北省工业发酵协同创新中心，湖北省工业微生物重点实验室，湖北工业大学生物工程与食品学院，湖北 武汉 430068
Author(s):
 YAN Ziyun;  CHEN Xiong;  LI Xin
 Key Laboratory of Fermentation Engineering of the Ministry of Education,Hubei Provincial Cooperative Innovation Center of Industrial Fermentation,Hubei Key Laboratory of Industrial Microbiology,Hubei Univ. of Tech., Wuhan 430068 China
关键词:
 ; 巴氏醋杆菌;  微量元素;  响应面法;  营养盐
Keywords:
 ; acetobacter pasteurianus;  trace elements;  response surface methodology;  nutrients
分类号:
Q815
文献标志码:
A
摘要:
 从26种微量元素中筛选出5种对巴氏醋杆菌有促进作用的生长因子，分别为精氨酸、赖氨酸、胞嘧啶、谷氨酰胺和叶酸，均以0.10 g/L的添加量复配加入营养盐培养基中，最大生物量提高26%。进行单因素实验确定初优添加量。利用Plackett-Burman实验、最陡爬坡实验和响应面实验进一步优化培养基成分，证实营养盐、赖氨酸和叶酸对巴氏醋杆菌的生长影响显著。通过回归方程 (R2=96.42%) ，预测当培养基成分配比为营养盐5.0 g/L、精氨酸0.10 g/L、赖氨酸0.46 g/L、胞嘧啶0.70 g/L、谷氨酰胺0.20 g/L、叶酸0.16 g/L时，巴氏醋杆菌在体积分数为9%的乙醇和1%的乙酸胁迫条件下的最大生物量可达0.45 g/L。经过3次平行验证试验，最大生物量为0.45 g/L，比未优化前的营养盐培养基提高了32%，与模型预测结果相吻合。
Abstract:
 From 26 kinds of trace elements, 5 kinds of factors that can promote the growth of Acetobacter pasteurianus were selected, namely arginine, lysine, cytosine, glutamine and folic acid, all with 0.10 g/L. The added amount was compounded and added to the nutrient medium, and the maximum biomass increased by 26%. A single factor experiment was carried out to determine the initial optimal addition amount, and the Plackett Burman experiment, the steepest climbing experiment and the response surface experiment were used to further optimize the medium composition. It was confirmed that nutrients, lysine and folic acid had a significant effect on the growth of Acetobacter pasteurianus. Through the regression equation (R2=96.42%), it was predicted that when the medium composition ratio is 5.0 g/L, arginine 0.10 g/L, lysine 0.46 g/L, cytosine 0.70 g/L, and glutamine with 0.20 g/L and 0.16 g/L folic acid, the maximum biomass of Acetobacter pasteurium can reach 0.45 g/L under the stress of 9% (V/V) ethanol and 1% (V/V) acetic acid. After 3 parallel verification experiments, the maximum biomass was 0.45 g/L, which was 32% higher than the nutrient medium before optimization, which was consistent with the model prediction results.

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备注/Memo

备注/Memo:
 ［收稿日期］ 2020-10-17
［第一作者］ 严子云(1994-)， 男， 湖北宜昌人，湖北工业大学硕士研究生，研究方向为微生物发酵
［通信作者］ 李 欣(1979-)， 男， 湖北荆州人，湖北工业大学讲师，研究方向为微生物发酵
更新日期/Last Update: 2021-04-23