[1]褚金磊,李 欣,王 志,等.富含巯基化合物酿酒酵母的筛选及发酵培养基优化[J].湖北工业大学学报,2019,34(5):32-36.
　CHU Jinlei,LI Xin,WANG Zhi,et al.Screening of High Intracellular Thiol Compounds Saccharomyces Cerevisiae and Optimization of Fermentation Medium[J].,2019,34(5):32-36.
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富含巯基化合物酿酒酵母的筛选及发酵培养基优化()
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《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
34卷
期数:
2019年第5期
页码:
32-36
栏目:
湖北工业大学学报
出版日期:
2019-10-30

文章信息/Info

Title:
Screening of High Intracellular Thiol Compounds Saccharomyces Cerevisiae and Optimization of Fermentation Medium
文章编号:
1003-4684(2019)05-0032-05
作者:
褚金磊;  李  欣;  王  志;  代  俊;  陈  雄
湖北工业大学生物工程与食品学院，发酵工程教育部重点试验室，工业发酵湖北省协同创新中心， 湖北 武汉 430068
Author(s):
CHU Jinlei;  LI Xin;  WANG Zhi;  DAI Jun;  CHEN Xiong
Key Laboratory of Ministry of Education for Fermentation Engineering, Hubei Provincial Cooperative Innovation Center for Industrial Fermentation, School of Biological Engineering and Food Science, Hubei Univ, of Tech., Wuhan 430068,China
关键词:
酿酒酵母;  巯基含量测定;  培养基优化;  响应面法
Keywords:
saccharomyces cerevisiae;  intracellular thiol content;  medium optimization;  response surface methodology
分类号:
TQ920.6
文献标志码:
A
摘要:
从实验室保藏的菌种中筛选出一株富含巯基化合物酿酒酵母YC-5，在初始培养基中胞内巯基含量最高为1.14%。采用响应面法对酿酒酵母YC5的培养基配方进行优化，建立糖蜜、蛋白胨和(NH4)2SO4用量的二次回归模型，确定培养基最佳配方为：糖蜜，70 mL/L；酵母浸粉，5 g/L；蛋白胨，8 g/L；MgSO4·7H2O，0.8 g/L；KH2PO4，0.6 g/L；(NH4)2SO4，0.2 g/L。在通过响应面法优化后的最适培养基中发酵12 h，胞内巯基含量最高可达1.82%，比未优化前提高了60%。
Abstract:
A strain of Saccharomyces cerevisiae YC5 which was rich in the sulfhydryl was screened from the strains preserved in laboratory. The highest intracellular thiol content was 1.14% in the initial medium. The response surface method was used to optimize the medium formula of Saccharomyces cerevisiae YC5, and a quadratic regression model of molasses, peptone and (NH4)2 SO4 dosage was established. The optimal formula for determining the medium was set as follows: molasses 70 mL/L, yeast extract 5 g/L, peptone 8 g/L, MgSO4·7H2O 0.8 g/L、KH2PO4 0.6 g/L、(NH4)2 SO4 0.2 g/L. After 12 h of fermentation in the optimum medium optimized by response surface methodology, the intracellular sulfhydryl content was as high as to 1.82%, which was 60% higher than that before optimization.

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备注/Memo

备注/Memo:
［收稿日期］ 2019-03-13
［第一作者］ 褚金磊(1993-)， 男， 湖北应城人，湖北工业大学硕士研究生，研究方向为发酵工程
［通信作者］ 陈   雄(1969-)， 男， 湖北荆门人，工学博士，湖北工业大学教授，研究方向为酵母工程
更新日期/Last Update: 2019-11-21