[1]刘凯文１,２,冯 敏３,等.茯苓对大鼠肾源性水肿的影响[J].湖北工业大学学报,2024,39(4):31-36.
　LIU Kaiwen,FENG Min,CHENG Wenhua,et al.Preliminary Study on the Effect of Poria Cocoson Nephrogenic Oedema in Rats[J].,2024,39(4):31-36.
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茯苓对大鼠肾源性水肿的影响()
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《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
39
期数:
2024年第4期
页码:
31-36
栏目:
出版日期:
2024-08-28

文章信息/Info

Title:
Preliminary Study on the Effect of Poria Cocoson Nephrogenic Oedema in Rats
文章编号:
１００３－４６８４(２０２４)０４-００３１-０６
作者:
刘凯文１; ２; 冯 敏３; 程文华３; 孙凡中３; 张军林１; ２
１ 湖北工业大学生物工程与食品学院,湖北 武汉 ４３００６８; ２ 武汉生物工程学院应用生物技术研究中心,湖北 武汉 ４３０４１５; ３ 湖北省疾病预防控制中心应用毒理湖北省重点实验室,湖北 武汉 ４３００７
Author(s):
LIU Kaiwen1; 2;  FENG Min3; CHENG Wenhua3;  SUN Fanzhong3;  ZHANG Junlin1; 2
1 School of Biological Engineering and Food Science, Hubei Univ. of Tech., Wuhan 430068, China;2 Center of Applied Biotechnology, Wuhan University of Bioengineering,Wuhan 430415, China;3 Hubei Provincial Key Laboratory for Applied Toxicology,Wuhan 430070, China
关键词:
茯苓; 肾源性水肿; 白介素; 利钠肽
分类号:
R２８
文献标志码:
A
摘要:
为研究茯苓对大鼠肾源性水肿的影响,探讨茯苓治疗肾源性水肿的可能机制,将１５０只 SD 级大鼠随机 分为对照组(１２只)和模型组(１３８只),使用阿霉素对模型组大鼠建立肾源性水肿模型.随机将肾源性水肿模型组 大鼠分为非给药模型组,茯苓粉(生、熟、代培)低、高剂量组,茯苓醇提物低、中、高剂量组,茯苓水提物低、中、高剂 量组.每组８只,雌雄各半,连续２１d灌胃给药.第０,７,１４,２１天记录体重变化,第２１天取血,通过 Elisa实验检 测大鼠血清ILＧ２、ILＧ６、ANP及 BNP水平.结果发现,茯苓粉(生、熟、代培)对于水肿程度的缓解(体重)和尿蛋白 的降低表现出一定作用.茯苓醇提物对于肾源性水肿大鼠血清 ANP、ILＧ６的降低效果最为显著,５种茯苓样品都 可以降低肾源性水肿大鼠血清BNP、ILＧ２的水平.茯苓可以缓解大鼠肾源性水肿,并降低大鼠血清 ANP、BNP、ILＧ ２、ILＧ６的水平,其中高剂量的熟茯苓对肾源性水肿大鼠尿蛋白缓解效果最好,茯苓醇提物对于肾源性水肿大鼠血 清 ANP、ILＧ６的降低效果最为显著,说明茯苓对肾源性水肿的治疗具有一定的积极作用.
Abstract:
This paper aims to study the effect of Poria cocos on nephrogenic edema in rats, and to explore the possible mechanism of its treatment. 150 SD rats were randomly divided into control group (12) and model group (138), and the model was established using doxorubicin. Rats with renal oedema were randomly divided into nonadministered model group, with low and high doses of Poria cocos powder (raw, cooked, and cultured), low, medium and high alcohol extract, and 8 in each group, half of each male and female, for 21d continuously. Weight changes were recorded on day 0,7,14,21, and blood was harvested on day 21, and serum IL2, IL6, ANP, and BNP levels were measured by the Elisa assay. Results show that Poria cocos powder (raw, cooked, and subcultured) had a certain effect on the reduction of oedema degree (weight) and urinary protein, among which cooked Poria cocos powder has the best effect. Poria cocos alcohol extract had the most significant effect on reducing serum ANP and IL6 in rats with nephrogenic oedema, and all five Poria cocos samples could reduce the levels of serum BNP and IL2 in rats with nephrogenic oedema. Poria cocos can relieve nephrogenic edema in rats and reduce the levels of serum ANP, BNP, and IL2 and IL6. Among them, high dose of cooked Poria cocos had the best effect on relieving urinary protein in rats with nephrogenic edema, and Poria alcohol extract had the most obvious effect on reducing serum ANP and IL6 in rats with nephrogenic edema. indicating that it has a positive effect in the treatment of nephrogenic edema.

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备注/Memo

备注/Memo:
[收稿日期]２０２２ ０８ ３１ [基金项目]国家重点研发计划(２０１７YFC１７０３００５) [第一作者]刘凯文(１９９５—),男,江西遂川人,湖北工业大学和武汉生物工程学院联合培养硕士研究生,研究方向为药效学. [通信作者]张军林(１９８１—),男,陕西宝鸡人,武汉生物工程学院副教授,研究方向为生物活性抗癌肽分离鉴定及基因治疗病毒载体表达 技术.
更新日期/Last Update: 2024-08-23