[1]张宏宏,叶 晨,张晓昀,等. 一株弯曲固氮菌对多环芳烃的降解特性研究[J].湖北工业大学学报,2023,(1):62-66.
　ZHANG Honghong,YE Chen,ZHANG Xiaoyun,et al. Degradation Characteristics of Polycyclic Aromatic Hydrocarbons by Azoarcus sp. CE3[J].,2023,(1):62-66.
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 一株弯曲固氮菌对多环芳烃的降解特性研究()
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《湖北工业大学学报》[ISSN:1003-4684/CN:42-1752/Z]

卷:
期数:
2023年第1期
页码:
62-66
栏目:
湖北工业大学学报
出版日期:
2023-03-13

文章信息/Info

Title:
 Degradation Characteristics of Polycyclic Aromatic Hydrocarbons by Azoarcus sp. CE3
文章编号:
1003-4684(2023)01-0062-05
作者:
 张宏宏1;  叶 晨1;  张晓昀2;  孟 恬1;  王黎明2;  黄玉屏1
 1 武汉大学生命科学学院，湖北 武汉 430072；
 2 湖北环境修复与治理技术研究有限公司， 湖北 黄石 435000
Author(s):
 ZHANG Honghong1;  YE Chen1;  ZHANG Xiaoyun2;  MENG Tian1;  WANG Liming2;  HUANG Yuping1
 1 College of Life Sciences, Wuhan University, Wuhan 430072, China;
 2 Hubei Environmental Remediation & Governance[JZ]Techonlogical Research Co., ltd.,Huangshi 435000,China
关键词:
 ; 多环芳烃;  荧蒽;  芘;  降解;  弯曲固氮菌
Keywords:
 ; polycyclic aromatic hydrocarbons (PAHs);  fluoranthene;  pyrene;  degradation;  Azoarcus
分类号:
Q939.99; X53
文献标志码:
A
摘要:
 微生物修复在多环芳烃（polycyclic aromatic hydrocarbons, PAHs）污染治理方面有着优良特性。为了获得高效降解PAHs的微生物，以荧蒽为唯一碳源进行筛选，分离得到一株高效降解细菌，命名为CE3。经16S rDNA序列分析法鉴定，菌株CE3属于弯曲固氮菌属（Azoarcus），这是首次发现该属菌株对高分子量PAHs具有降解能力。菌株CE3除了能降解荧蒽，还能降解苯、菲、芘、荧蒽、苯并[a]蒽、3-4苯并芘和苯并[b]荧蒽，且对荧蒽和芘的降解率较高，均达到50%以上。分析各种条件对CE3菌降解荧蒽和芘的影响，发现CE3菌能在较广的温度和pH范围降解荧蒽或芘，并且添加酵母提取物、蔗糖和果糖可使CE3菌降解能力提高。
Abstract:
 Microbial remediation has excellent characteristics in the control of polycyclic aromatic hydrocarbons (PAHs) pollution. In order to obtain microorganisms that can efficiently degrade PAHs, fluoranthene was used as the only carbon source to screen microorganisms. And then a high-efficiency degrading bacterium named CE3 was isolated. Strain CE3 was identified to belong to Azoarcus genus based on its 16S rDNA sequence analysis. To our knowledge, this is the first report that the strain of the Azoarcus genus has the ability to degrade high molecular weight PAHs. In addition to fluoranthene, strain CE3 also could degrade benzene, phenanthrene, pyrene, fluoranthene, benzo [a] anthracene, benzo [3,4] pyrene and benzo [b] fluoranthene. The degradation rates of fluoranthene and pyrene are more than 50% and higher than the degradation rates of other PAHs. Then, the degradation rates of fluoranthene and pyrene by strain CE3 under various conditions were analyzed in detail by HPLC method. The results showed that Azoarcus sp. CE3 could degrade fluoranthene or pyrene in a wide range of temperature and pH; moreover, the addition of yeast extract, sucrose and fructose to the medium could improve its degradation ability.

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备注/Memo

备注/Memo:
［收稿日期］ 2021-09-18
［基金项目］ 湖北环境修复与治理技术研究有限公司与武汉大学合作项目（HER-GCZX-CG-2018-05）
［第一作者］ 张宏宏（1994-），女，湖北武汉人，武汉大学硕士研究生，研究方向为微生物学
［通信作者］ 黄玉屏（1968-），女，湖北武汉人，工学博士，武汉大学副教授，研究方向为微生物学
更新日期/Last Update: 2023-03-14